Abstract: Objective　A meta-analysis was conducted to study the effects of human milk oligosaccharides (HMOs) on necrotizing enterocolitis (NEC).Methods　PubMed, Embase, Web of Science, Cochrane library, OVID, Scopus, ProQuest, CNKI, Wanfang database, Weipu Periodical Database, Chinese Medical Journal Database, Chinese Biomedical Literature Database were searched for the randomized controlled animal studies of the effects of HMOs on animal models of NEC published up to August 2024. SYRCLE risk bias assessment tool was used to evaluate literature quality, and the RevMan 5.4 software was used for meta-analysis.Results　After strict inclusion and exclusion screening, a total of 6 studies were included, with a total of 175 animal models.The result of meta-analysis showed that compared with the control group, the NEC animal model in the HMOs group had lower mortality (RR=0.44,95%CI 0.24-0.81,P<0.01) and intestinal histopathological scores(MD=-1.44,95%CI -1.66--1.22,P< 0.001).Supplementation of disialyllacto-N-tetraose (DSLNT) alone could reduce NEC mortality (RR= 0.41,95%CI 0.17-0.97,P<0.05) and intestinal histopathological scores (MD=-1.54,95%CI -1.85--1.24,P<0.001).Conclusion　HMOs can reduce the mortality and intestinal histopathological scores associated with NEC animal models.

Key words: human milk oligosaccharides, necrotizing enterocolitis, animal models, Meta-analysis

摘要： 目的　通过Meta分析研究母乳低聚糖(HMOs)对坏死性小肠结肠炎(NEC)的影响。方法　计算机检索 PubMed、Embase、Web of Science、Cochrane library、OVID、Scopus、ProQuest、中国知网、万方数据库、维普、中华医学期 刊全文库及中国生物医学文献数据库,筛选关于HMOs对NEC动物模型影响的随机对照实验,检索时间范围为建 库起至2024年8月份。采用SYRCLE风险偏倚评估工具进行文献质量评价,应用RevMan 5.4软件进行Meta分 析。结果　经过严格的纳入和排除筛选,共纳入6项研究,总计动物模型175个。Meta分析结果显示,与对照组相 比,HMOs组的NEC动物模型具有更低的死亡率(RR=0.44,95%CI 0.24~0.81,P<0.01)和肠道组织病理学评分 (MD=-1.44,95%CI -1.66~-1.22,P<0.001)。单独添加二唾液酸-N-四糖(disialyllacto-N-tetraose, DSLNT)可降 低NEC死亡率(RR=0.41,95%CI 0.17~0.97,P<0.05)及肠道组织病理学评分(MD=-1.54,95%CI -1.85~-1.24,P<0.001)。结论　HMOs可降低NEC动物模型相关病死率及肠道组织病理学评分。

关键词: 母乳低聚糖, 坏死性小肠结肠炎, 动物模型, Meta分析